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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- bs-0033R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-0033R, RRID:AB_10855396
- Product name
- P53 protein(wt-p53) Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic peptide derived from human P53
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Submitted references Targeted endothelial gene deletion of triggering receptor expressed on myeloid cells-1 protects mice during septic shock.
Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats.
Transcription of HOTAIR is regulated by RhoC-MRTF-A-SRF signaling pathway in human breast cancer cells.
Increased levels of CCR7(lo)PD-1(hi) CXCR5+ CD4+ T cells, and associated factors Bcl-6, CXCR5, IL-21 and IL-6 contribute to repeated implantation failure.
Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis.
Jolly L, Carrasco K, Derive M, Lemarié J, Boufenzer A, Gibot S
Cardiovascular research 2018 May 1;114(6):907-918
Cardiovascular research 2018 May 1;114(6):907-918
Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats.
Yang H, Wu S, Feng R, Huang J, Liu L, Liu F, Chen Y
Stem cell research & therapy 2017 Nov 21;8(1):267
Stem cell research & therapy 2017 Nov 21;8(1):267
Transcription of HOTAIR is regulated by RhoC-MRTF-A-SRF signaling pathway in human breast cancer cells.
He H, Wei Z, Du F, Meng C, Zheng D, Lai Y, Yao H, Zhou H, Wang N, Luo XG, Ma W, Zhang TC
Cellular signalling 2017 Feb;31:87-95
Cellular signalling 2017 Feb;31:87-95
Increased levels of CCR7(lo)PD-1(hi) CXCR5+ CD4+ T cells, and associated factors Bcl-6, CXCR5, IL-21 and IL-6 contribute to repeated implantation failure.
Gong Q, Zhu Y, Pang N, Ai H, Gong X, La X, Ding J
Experimental and therapeutic medicine 2017 Dec;14(6):5931-5941
Experimental and therapeutic medicine 2017 Dec;14(6):5931-5941
Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis.
Chen J, Shi D, Liu X, Fang S, Zhang J, Zhao Y
BMC cancer 2012 Oct 10;12:464
BMC cancer 2012 Oct 10;12:464
No comments: Submit comment
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- 1 rat brain, L2 human colon carcinoma lysates probed (bs-0033R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band and observed band size: 53kDa.
- Sample type
- Human
- Other comments
- Colon
Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded human ovarian carcinoma labeled with Rabbit Anti-P53 protein(wt-p53) Polyclonal Antibody (bs-0033R), Unconjugated 1:300 followed by conjugation to the secondary antibody and DAB staining
- Sample type
- Human
- Other comments
- Ovarian
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of p53 protein in mouse squamous cell cancer (KLN-205 cell line) with BS-0033R antibody (1:50) and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
- Sample type
- Mouse
