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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0562 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0562, RRID:AB_982774
- Product name
- TP53 (phospho S315) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of TP53.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The multiple roles of p53 in the pathogenesis of HIV associated dementia.
p53-A pro-apoptotic signal transducer involved in AIDS.
Guilt by association? p53 and the development of aneuploidy in cancer.
Garden GA, Morrison RS
Biochemical and biophysical research communications 2005 Jun 10;331(3):799-809
Biochemical and biophysical research communications 2005 Jun 10;331(3):799-809
p53-A pro-apoptotic signal transducer involved in AIDS.
Castedo M, Perfettini JL, Piacentini M, Kroemer G
Biochemical and biophysical research communications 2005 Jun 10;331(3):701-6
Biochemical and biophysical research communications 2005 Jun 10;331(3):701-6
Guilt by association? p53 and the development of aneuploidy in cancer.
Duensing A, Duensing S
Biochemical and biophysical research communications 2005 Jun 10;331(3):694-700
Biochemical and biophysical research communications 2005 Jun 10;331(3):694-700
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TP53 (phospho S315) polyclonal antibody (Cat # PAB0562). Western blot analysis of TP53 expression in Jurkat.
- Validation comment
- Western Blot (Cell lysate)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with TP53 (phospho S315) polyclonal antibody (Cat # PAB0562) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of TP53 (phospho S315) polyclonal antibody (Cat # PAB0562) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
