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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [3]
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- Product number
- TA502780 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502780, RRID:AB_11128598
- Product name
- TP53 mouse monoclonal antibody, clone OTI3D5 (formerly 3D5)
- Antibody type
- Monoclonal
- Description
- TP53 mouse monoclonal antibody, clone OTI3D5 (formerly 3D5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TP53
- Isotype
- IgG
- Antibody clone number
- OTI3D5
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Decreased sirtuin 4 expression is associated with poor prognosis in patients with invasive breast cancer.
Shi Q, Liu T, Zhang X, Geng J, He X, Nu M, Pang D
Oncology letters 2016 Oct;12(4):2606-2612
Oncology letters 2016 Oct;12(4):2606-2612
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-TP53 mouse monoclonal antibody (TA502780) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53(RC200003).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502780)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502780)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200003 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TP53 antibody(TA502780), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-TP53 antibody(TA502780),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody(TA502780),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
