M00001-4

antibody from Boster Biological Technology

Targeting: TP53 LFS1, p53

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00001-4

Provider

Boster Biological Technology

Product name

Anti-p53 Monoclonal Antibody (PAb122)

Antibody type

Monoclonal

Description

Mouse Monoclonal pAb122 antibody for Tp53 detection. Tested positive for Flow Cytometry, IF, IHC, IP, WB in Human, Mouse, Rat

Reactivity

Human, Mouse, Rat

Host

Mouse

Antibody clone number

pAb122

Vial size

200µg

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

-20°C

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Tp53 using anti-Tp53 antibody (M00001-4).Tp53 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Tp53 Antibody (M00001-4) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Additional image