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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- 200-301-174 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-174, RRID:AB_2206455
- Product name
- Anti-p53 (MOUSE) Monoclonal Antibody - 200-301-174
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Immunohistochemistry of Mouse anti-p53 antibody. Tissue: human brain. Fixation: free-floating. Antigen retrieval: not required. Primary antibody: anti-human-p53 antibody at 1:500 for 1 h at RT. Co-stained with YFP and Sox2 antibodies. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: p53 is nuclear and cytoplasmic. Staining: p53 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. z?stacks from confocal expressed as one composite focal plain.
- Sample type
- IF Microscopy
