3340

antibody from QED Bioscience

Targeting: TP53 LFS1, p53

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

3340

Provider

QED Bioscience

Product name

Anti-p53 Antibody (3340)

Antibody type

Monoclonal

Description

Anti-p53 Mouse Monoclonal Antibody

Reactivity

Human

Host

Mouse

Epitope

Gel-purified p53 containing aa 14-389 of human p53.

Isotype

IgG

Antibody clone number

240

Vial size

100ug

Concentration

Lot Specific

Storage

This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.

Handling

ELISA, FACS, IHC (frozen and paraffin), Immunoprecipitation, Immunoblotting. IHC: use at 2-4ug/ml. Extensive washing may be necessary. Protein digestion and/or microwave antigen retrieval prior to staining is not required. #3340 on paraffin-embedded HBL435 xenograft. Immunoprecipitation: use at 10ug/mg of lysate. This antibody reacts only with mutant p53 under non-denaturing conditions. Immunoblotting: use at 5-10ug/ml. A band of 53kDa is detected. #3340 on 30ug of 293T (A) and A431 (B) cell lysates. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.