614202

antibody from BioLegend

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

614202

Provider

BioLegend

Product name

Purified anti-p53-Acetylated (Lys382)

Antibody type

Polyclonal

Antigen

Modified peptide

Description

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73. The Poly6142 antibody recognizes human acetylated p53 (Lys382) and has been shown to be useful for Western blotting.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

200 µl

Storage

Upon receipt, store frozen at -20° C.

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

MCF-7 cells were treated with 50 µM sodium butyrate and 2 nM actinomycin D for 8 hrs. MCF7 cell extract was resolved by electrophoresis (panel B) or immunoprecipitated with a p53 monoclonal (panel A) and resolved by electrophoresis. Proteins were transferred to nitrocellulose, and probed with rabbit polyclonal antibody against acetylated p53 (Lys382). Lane 1=untreated, control MCF-7 cells; Lane 2=treated MCF-7 cells. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.

Conjugate

Unconjugated