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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AB0065-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0065-200, RRID:AB_2333152
- Product name
- Anti-P53
- Antibody type
- Polyclonal
- Description
- REP1 is the component A of the RAB geranylgeranyl transferase holoenzyme. In the dimeric holoenzyme, this subunit binds unprenylated Rab GTPases and then presents them to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. Mutations in this protein are a cause of a type of retinal degeneration, called choroideremia.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 600 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell lysates at 50 µg per lane
- Sample type
- 293HEK cell transfected with GFP-P53
- Primary Ab dilution
- 1/500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Lysates at 50 µg per lane
- Sample type
- HaCat and SRA cell lines
- Primary Ab dilution
- 1/2,500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- cells were fixed with methanol
- Sample type
- Hepa1-6 cell line
- Primary Ab dilution
- 1/50
- Conjugate
- Unconjugated
