NB200-103

antibody from Novus Biologicals

Targeting: TP53 LFS1, p53

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-103

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-103, RRID:AB_10001083

Product name

Mouse Monoclonal p53 Antibody

Antibody type

Monoclonal

Description

Protein G purified. This monoclonal recognizes both mutant forms and wild-type human p53 under denaturing conditions. It does not recognize nondenatured wild type p53. In nondenaturing conditions (IP) it only recognizes mutant p53.

Reactivity

Human, Mouse, Rat, Chicken/Avian, Xenopus, Yeast

Host

Mouse

Antigen sequence

The epitope has been mapped between
amino acids 213 and 217 on human p
53. The epitiope may be conformatio
nal as it reacts with mutant p53 in
nondenatured form.


Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Autophagy promotes mammalian survival by suppressing oxidative stress and p53.

Yang Y, Karsli-Uzunbas G, Poillet-Perez L, Sawant A, Hu ZS, Zhao Y, Moore D, Hu W, White E
Genes & development 2020 May 1;34(9-10):688-700

---

Intermittent fasting benefits on alpha- and beta-cell arrangement in diet-induced obese mice pancreatic islet.

Marinho TS, Borges CC, Aguila MB, Mandarim-de-Lacerda CA
Journal of diabetes and its complications 2020 Mar;34(3):107497

---

Dexmedetomidine alleviated sepsis‑induced myocardial ferroptosis and septic heart injury.

Wang C, Yuan W, Hu A, Lin J, Xia Z, Yang CF, Li Y, Zhang Z
Molecular medicine reports 2020 Jul;22(1):175-184

---

A Molecular Signature in Blood Reveals a Role for p53 in Regulating Malaria-Induced Inflammation.

Tran TM, Guha R, Portugal S, Skinner J, Ongoiba A, Bhardwaj J, Jones M, Moebius J, Venepally P, Doumbo S, DeRiso EA, Li S, Vijayan K, Anzick SL, Hart GT, O'Connell EM, Doumbo OK, Kaushansky A, Alter G, Felgner PL, Lorenzi H, Kayentao K, Traore B, Kirkness EF, Crompton PD
Immunity 2019 Oct 15;51(4):750-765.e10

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PM_2.5-induced ADRB2 hypermethylation contributed to cardiac dysfunction through cardiomyocytes apoptosis via PI3K/Akt pathway.

Yang X, Zhao T, Feng L, Shi Y, Jiang J, Liang S, Sun B, Xu Q, Duan J, Sun Z
Environment international 2019 Jun;127:601-614

---

Investigation of platinum nanoparticle properties against U87 glioblastoma multiforme.

Kutwin M, Sawosz E, Jaworski S, Hinzmann M, Wierzbicki M, Hotowy A, Grodzik M, Winnicka A, Chwalibog A
Archives of medical science : AMS 2017 Oct;13(6):1322-1334

---

Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures.

Graham NA, Minasyan A, Lomova A, Cass A, Balanis NG, Friedman M, Chan S, Zhao S, Delgado A, Go J, Beck L, Hurtz C, Ng C, Qiao R, Ten Hoeve J, Palaskas N, Wu H, Müschen M, Multani AS, Port E, Larson SM, Schultz N, Braas D, Christofk HR, Mellinghoff IK, Graeber TG
Molecular systems biology 2017 Feb 15;13(2):914

---

Renal Protective Effects of 17β-Estradiol on Mice with Acute Aristolochic Acid Nephropathy.

Shi M, Ma L, Zhou L, Fu P
Molecules (Basel, Switzerland) 2016 Oct 18;21(10)

---

Mitochondrial catalase suppresses naturally occurring lung cancer in old mice.

Ge X, Pettan-Brewer C, Morton J, Carter K, Fatemi S, Rabinovitch P, Ladiges WC
Pathobiology of aging & age related diseases 2015;5:28776

---

E2F1 responds to ultraviolet radiation by directly stimulating DNA repair and suppressing carcinogenesis.

Biswas AK, Mitchell DL, Johnson DG
Cancer research 2014 Jun 15;74(12):3369-77

---

Dual role for mammalian DNA polymerase ζ in maintaining genome stability and proliferative responses.

Lange SS, Bedford E, Reh S, Wittschieben JP, Carbajal S, Kusewitt DF, DiGiovanni J, Wood RD
Proceedings of the National Academy of Sciences of the United States of America 2013 Feb 19;110(8):E687-96

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Reduced hepatic tumor incidence in cyclin G1-deficient mice.

Jensen MR, Factor VM, Fantozzi A, Helin K, Huh CG, Thorgeirsson SS
Hepatology (Baltimore, Md.) 2003 Apr;37(4):862-70

---

Regulation of p53 stability and p53-dependent apoptosis by NADH quinone oxidoreductase 1.

Asher G, Lotem J, Cohen B, Sachs L, Shaul Y
Proceedings of the National Academy of Sciences of the United States of America 2001 Jan 30;98(3):1188-93

---

Cisplatin- and paclitaxel-induced apoptosis of ovarian carcinoma cells and the relationship between bax and bak up-regulation and the functional status of p53.

Jones NA, Turner J, McIlwrath AJ, Brown R, Dive C
Molecular pharmacology 1998 May;53(5):819-26

---

Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90.

Blagosklonny MV, Toretsky J, Bohen S, Neckers L
Proceedings of the National Academy of Sciences of the United States of America 1996 Aug 6;93(16):8379-83

---

Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90.

Blagosklonny MV, Toretsky J, Bohen S, Neckers L
Proceedings of the National Academy of Sciences of the United States of America 1996 Aug 6;93(16):8379-83

---

Wild-type p53 adopts a 'mutant'-like conformation when bound to DNA.

Halazonetis TD, Davis LJ, Kandil AN
The EMBO journal 1993 Mar;12(3):1021-8

---

A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity when generated from wild-type but not from oncogenic mutant p53 protein.

Bargonetti J, Manfredi JJ, Chen X, Marshak DR, Prives C
Genes & development 1993 Dec;7(12B):2565-74

---

A proteolytic fragment from the central region of p53 has marked sequence-specific DNA-binding activity when generated from wild-type but not from oncogenic mutant p53 protein.

Bargonetti J, Manfredi JJ, Chen X, Marshak DR, Prives C
Genes & development 1993 Dec;7(12B):2565-74

---

Accumulation of wild type p53 protein in human astrocytomas.

Rubio MP, von Deimling A, Yandell DW, Wiestler OD, Gusella JF, Louis DN
Cancer research 1993 Aug 1;53(15):3465-7

---

Increased p53 protein content of colorectal tumours correlates with poor survival.

Remvikos Y, Tominaga O, Hammel P, Laurent-Puig P, Salmon RJ, Dutrillaux B, Thomas G
British journal of cancer 1992 Oct;66(4):758-64

---

Increased p53 protein content of colorectal tumours correlates with poor survival.

Remvikos Y, Tominaga O, Hammel P, Laurent-Puig P, Salmon RJ, Dutrillaux B, Thomas G
British journal of cancer 1992 Oct;66(4):758-64

---

Frequent p53 mutations in head and neck cancer.

Somers KD, Merrick MA, Lopez ME, Incognito LS, Schechter GL, Casey G
Cancer research 1992 Nov 1;52(21):5997-6000

---

Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma.

Eccles DM, Brett L, Lessells A, Gruber L, Lane D, Steel CM, Leonard RC
British journal of cancer 1992 Jan;65(1):40-4

---

Overexpression of the p53 protein and allele loss at 17p13 in ovarian carcinoma.

Eccles DM, Brett L, Lessells A, Gruber L, Lane D, Steel CM, Leonard RC
British journal of cancer 1992 Jan;65(1):40-4

---

Expression of p53 and 17p allelic loss in colorectal carcinoma.

Cunningham J, Lust JA, Schaid DJ, Bren GD, Carpenter HA, Rizza E, Kovach JS, Thibodeau SN
Cancer research 1992 Apr 1;52(7):1974-80

---

p53 is frequently mutated in Burkitt's lymphoma cell lines.

Farrell PJ, Allan GJ, Shanahan F, Vousden KH, Crook T
The EMBO journal 1991 Oct;10(10):2879-87

---

Genetic basis for p53 overexpression in human breast cancer.

Davidoff AM, Humphrey PA, Iglehart JD, Marks JR
Proceedings of the National Academy of Sciences of the United States of America 1991 Jun 1;88(11):5006-10

---

Genetic basis for p53 overexpression in human breast cancer.

Davidoff AM, Humphrey PA, Iglehart JD, Marks JR
Proceedings of the National Academy of Sciences of the United States of America 1991 Jun 1;88(11):5006-10

---

Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein.

Martinez J, Georgoff I, Martinez J, Levine AJ
Genes & development 1991 Feb;5(2):151-9

---

Cellular localization and cell cycle regulation by a temperature-sensitive p53 protein.

Martinez J, Georgoff I, Martinez J, Levine AJ
Genes & development 1991 Feb;5(2):151-9

---

Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form.

Gannon JV, Greaves R, Iggo R, Lane DP
The EMBO journal 1990 May;9(5):1595-602

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on MCF7 cells with p53 [PAb 240] Antibody NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.