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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00079084-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00079084-M01, RRID:AB_464092
- Product name
- WDR77 monoclonal antibody (M01), clone 3F10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant WDR77.
- Antigen sequence
MRKETPPPLVPPAAREWNLPPNAPACMERQLEAAR
YRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNE
GFCSAGVQTEAGVADLTWVGERGILVASDSGAVEL
WELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQA
VSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAA
SPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSA
PGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKST
SCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSL
AVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTT
VGWDHQVVHHVVPTEPLPAPGPASVTE- Isotype
- IgG
- Antibody clone number
- 3F10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references PRMT5 is upregulated in malignant and metastatic melanoma and regulates expression of MITF and p27(Kip1.).
Nicholas C, Yang J, Peters SB, Bill MA, Baiocchi RA, Yan F, Sïf S, Tae S, Gaudio E, Wu X, Grever MR, Young GS, Lesinski GB
PloS one 2013;8(9):e74710
PloS one 2013;8(9):e74710
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- WDR77 monoclonal antibody (M01), clone 3F10 Western Blot analysis of WDR77 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.Lane 1: WDR77 transfected lysate(36.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged WDR77 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
