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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00079084-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00079084-M01, RRID:AB_549158
- Product name
- Mouse Monoclonal WDR77 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. WDR77 - WD repeat domain 77
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references PRMT5 is upregulated in malignant and metastatic melanoma and regulates expression of MITF and p27(Kip1.).
Nicholas C, Yang J, Peters SB, Bill MA, Baiocchi RA, Yan F, Sïf S, Tae S, Gaudio E, Wu X, Grever MR, Young GS, Lesinski GB
PloS one 2013;8(9):e74710
PloS one 2013;8(9):e74710
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - Analysis of WDR77 expression in transfected 293T cell line by WDR77 monoclonal antibody (M01), clone 3F10.Lane 1: WDR77 transfected lysate(36.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WDR77 Antibody (3F10) [H00079084-M01] - WDR77 monoclonal antibody (M01), clone 3F10 Analysis of WDR77 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: WDR77 Antibody (3F10) [H00079084-M01] - Detection limit for recombinant GST tagged WDR77 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: WDR77 Antibody (3F10) [H00079084-M01] - Analysis of monoclonal antibody to WDR77 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
