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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- STJ99206 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-WDR77 antibody [8A10-C10-E8] (STJ99206)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Methylosome Protein 50 is suitable for use in Western Blot and Immunocytochemistry research applications.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of WDR77 in C6, 3T3 and K562 cell lysates using WDR77 mouse mAb (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100, next using WDR77 mouse mAb (dilution 1:100).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
