NB100-77305

antibody from Novus Biologicals

Targeting: WDR77 MEP50, p44

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-77305

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-77305, RRID:AB_1085984

Product name

Rabbit Polyclonal WDR77 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: WDR77 Antibody [NB100-77305] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-WDR77/MEP50 antibody sed for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WDR77/MEP50 was also immunoprecipitated by rabbit anti-WDR77/MEP50 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: WDR77 Antibody [NB100-77305] - Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti- WDR77/MEP50 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB