M02185

antibody from Boster Biological Technology

Targeting: ARRB1 ARR1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M02185

Provider

Boster Biological Technology

Product name

Anti-ARRB1/Beta Arrestin 1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for BETA ARRESTIN 1/ARRB1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat BETA ARRESTIN 1/ARRB1 information: Molecular Weight: 47066 MW; Subcellular Localization: Cytoplasm. Nucleus. Cell membrane. Membrane, clathrin-coated pit . Cell projection, pseudopodium . Cytoplasmic vesicle. Translocates to the plasma membrane and colocalizes with antagonist-stimulated GPCRs. The monomeric form is predominantly located in the nucleus. The oligomeric form is located in the cytoplasm. Translocates to the nucleus upon stimulation of OPRD1 (By similarity).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

CCB-1

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human kidney, using ARRB1 Antibody(M02185)ARRB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARRB1 Antibody (M02185)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image