PA5-87555

antibody from Invitrogen Antibodies

Targeting: ARRB1 ARR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87555

Provider

Invitrogen Antibodies

Product name

beta Arrestin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RKVQYAPERP GPQPTAETTR QFLMSDKPLH LEASLDKEIY YHGEPISVNV HVTNNTNKTV KKIKISVRQY ADICLFNTAQ YKCPVAMEEA DDTVAPSSTF CKVYTLTPFL ANNREKRGLA LDGKLKHEDT NLASSTLLRE GANREILGII VSYKVKVKLV VSRGGLLGDL ASSDVAVELP FTLMHPKPKE EPPHREVPEN ETPVDTNLIE LDTNDDDIVF EDFARQRLKG MKDDKEEEED GTGSPQLNNR; Positive Samples: U-937, K-562, Mouse brain, Mouse spleen; Cellular Location: Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus, clathrin-coated pit, pseudopodium

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.83 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of beta Arrestin 1 in extracts of various cell lines using beta Arrestin 1 Polyclonal Antibody (Product # PA5-87555) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of beta Arrestin 1 was performed in HeLa cells using beta Arrestin 1 Polyclonal Antibody (Product # PA5-87555).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of beta Arrestin 1 in paraffin-embedded rat heart using beta Arrestin 1 Polyclonal Antibody (Product # PA5-87555) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of beta Arrestin 1 in paraffin-embedded mouse spleen using beta Arrestin 1 Polyclonal Antibody (Product # PA5-87555) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of beta Arrestin 1 was performed in 200 µg extracts of K-562 cells using beta Arrestin 1 Polyclonal Antibody (Product # PA5-87555). Western blot was performed from the immunoprecipitate using beta Arrestin 1 Polyclonal Antibody.