GTX113306

antibody from GeneTex

Targeting: AIFM1 AIF, AUNX1, CMTX4, DFNX5, NAMSD, PDCD8

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX113306

Provider

GeneTex

Proper citation

GeneTex Cat#GTX113306, RRID:AB_2036190

Product name

AIF antibody [N1C1]

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Age-Dependent Up-Regulation of HCN Channels in Spiral Ganglion Neurons Coincide With Hearing Loss in Mice.

Shen H, Liu W, Geng Q, Li H, Lu M, Liang P, Zhang B, Yamoah EN, Lv P
Frontiers in aging neuroscience 2018;10:353

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Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway.

Lin SC, Chu PY, Liao WT, Wu MY, Tsui KH, Lin LT, Huang CH, Chen LL, Li CJ
Oncology reports 2018 Feb;39(2):703-710

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Pigment Epithelium-Derived Factor Mediates Autophagy and Apoptosis in Myocardial Hypoxia/Reoxygenation Injury.

Kuo HF, Liu PL, Chong IW, Liu YP, Chen YH, Ku PM, Li CY, Chen HH, Chiang HC, Wang CL, Chen HJ, Chen YC, Hsieh CC
PloS one 2016;11(5):e0156059

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Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.

Chen MF, Huang SJ, Huang CC, Liu PS, Lin KI, Liu CW, Hsieh WC, Shiu LY, Chen CH
BMC cancer 2016 Jul 26;16:532

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Pre-administration of BAX-inhibiting peptides decrease the loss of the nigral dopaminergic neurons in rats.

Ma C, Pan Y, Yang Z, Meng Z, Sun R, Wang T, Fei Y, Fan W
Life sciences 2016 Jan 1;144:113-20

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

40128

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX113306 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: H1299 B: Hep G2 (GTX27900) 7.5% SDS PAGE GTX113306 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Rat tissue extract (50 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AIF antibody [N1C1] (GTX113306) diluted at 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using AIF(GTX113306) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using AIF(GTX113306) antibody at 1:100 dilution.