H00246243-M01

antibody from Abnova Corporation

Targeting: RNASEH1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00246243-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00246243-M01, RRID:AB_530236

Product name

RNASEH1 monoclonal antibody (M01), clone 5D10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RNASEH1.

Antigen sequence

AACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQ
GWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQW
MHVPGHSGFIGNEEADRLAREGAKQSED


Isotype

IgG

Antibody clone number

5D10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RNASEH1 monoclonal antibody (M01), clone 5D10 Western Blot analysis of RNASEH1 expression in HeLa ( Cat # L013V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RNASEH1 expression in transfected 293T cell line by RNASEH1 monoclonal antibody (M01), clone 5D10.Lane 1: RNASEH1 transfected lysate(32.1 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RNASEH1 monoclonal antibody (M01), clone 5D10. Western Blot analysis of RNASEH1 expression in PC-12 ( Cat # L012V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RNASEH1 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol