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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 15606-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNASEH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSWLLFLAH RVALAALPCR RGSRGFGMFY AVRRGRKTGV FLTWNECRAQ VDRFPAARFK KFATEDEAWA FVRKSASPEV SEGHENQHGQ ESEAKASKRL REPLDGDGHE SAEPYAKHMK PSVEPAPPVS RDTFSYMGDF VVVYTDGCCS SNGRRRPRAG IGVYWGPGHP LNVGIRLPGR QTNQRAEIHA ACKAIEQAKT QNINKLVLYT DSMFTINGIT NWVQGWKKNG WKTSAGKEVI NKEDFVALER LTQGMDIQWM HVPGHSGFIG NEEADRLARE GAKQSED (1-286 aa encoded by BC002973)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15606-1-AP (RNASEH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 15606-1-AP (RNASEH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 15606-1-AP (RNASEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RNASEH1 (IP:15606-1-AP, 3ug; Detection:15606-1-AP 1:500) with HeLa cells lysate 1200ug.
