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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [20]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB729F-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCR3 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross-react with Y3 cells transfected with human CCR3.
- Reactivity
- Mouse
- Host
- Rat
- Antigen sequence
P51678- Isotype
- IgG
- Antibody clone number
- 83101
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Inhibition of soluble epoxide hydrolase attenuates eosinophil recruitment and food allergen-induced gastrointestinal inflammation.
Frontline Science: Eosinophil-deficient MBP-1 and EPX double-knockout mice link pulmonary remodeling and airway dysfunction with type 2 inflammation.
CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production.
Single-cell analysis of mixed-lineage states leading to a binary cell fate choice.
Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1.
Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge.
Generation of eosinophils from cryopreserved murine bone marrow cells.
Targeted ablation of miR-21 decreases murine eosinophil progenitor cell growth.
Eosinophil-expressed galectin-3 regulates cell trafficking and migration.
Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils.
Inflammation triggers emergency granulopoiesis through a density-dependent feedback mechanism.
SIRPĪ±/CD172a regulates eosinophil homeostasis.
N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation.
Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice.
CD28 and ICOS play complementary non-overlapping roles in the development of Th2 immunity in vivo.
Intravascular inactivation of CCR5 by n-Nonanoyl-CC chemokine ligand 14 and inhibition of allergic airway inflammation.
CD43 regulates Th2 differentiation and inflammation.
Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma.
A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation.
CD48 is an allergen and IL-3-induced activation molecule on eosinophils.
Bastan I, Ge XN, Dileepan M, Greenberg YG, Guedes AG, Hwang SH, Hammock BD, Washabau RJ, Rao SP, Sriramarao P
Journal of leukocyte biology 2018 Jul;104(1):109-122
Journal of leukocyte biology 2018 Jul;104(1):109-122
Frontline Science: Eosinophil-deficient MBP-1 and EPX double-knockout mice link pulmonary remodeling and airway dysfunction with type 2 inflammation.
Ochkur SI, Doyle AD, Jacobsen EA, LeSuer WE, Li W, Protheroe CA, Zellner KR, Colbert D, Shen HH, Irvin CG, Lee JJ, Lee NA
Journal of leukocyte biology 2017 Sep;102(3):589-599
Journal of leukocyte biology 2017 Sep;102(3):589-599
CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production.
Rozenberg P, Reichman H, Zab-Bar I, Itan M, Pasmanik-Chor M, Bouffi C, Qimron U, Bachelet I, Fulkerson PC, Rothenberg ME, Munitz A
Scientific reports 2017 Jul 19;7(1):5922
Scientific reports 2017 Jul 19;7(1):5922
Single-cell analysis of mixed-lineage states leading to a binary cell fate choice.
Olsson A, Venkatasubramanian M, Chaudhri VK, Aronow BJ, Salomonis N, Singh H, Grimes HL
Nature 2016 Sep 29;537(7622):698-702
Nature 2016 Sep 29;537(7622):698-702
Regulation of eosinophilia and allergic airway inflammation by the glycan-binding protein galectin-1.
Ge XN, Ha SG, Greenberg YG, Rao A, Bastan I, Blidner AG, Rao SP, Rabinovich GA, Sriramarao P
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 16;113(33):E4837-46
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 16;113(33):E4837-46
Eosinophils contribute to the resolution of lung-allergic responses following repeated allergen challenge.
Takeda K, Shiraishi Y, Ashino S, Han J, Jia Y, Wang M, Lee NA, Lee JJ, Gelfand EW
The Journal of allergy and clinical immunology 2015 Feb;135(2):451-60
The Journal of allergy and clinical immunology 2015 Feb;135(2):451-60
Generation of eosinophils from cryopreserved murine bone marrow cells.
Schollaert KL, Stephens MR, Gray JK, Fulkerson PC
PloS one 2014;9(12):e116141
PloS one 2014;9(12):e116141
Targeted ablation of miR-21 decreases murine eosinophil progenitor cell growth.
Lu TX, Lim EJ, Itskovich S, Besse JA, Plassard AJ, Mingler MK, Rothenberg JA, Fulkerson PC, Aronow BJ, Rothenberg ME
PloS one 2013;8(3):e59397
PloS one 2013;8(3):e59397
Eosinophil-expressed galectin-3 regulates cell trafficking and migration.
Ge XN, Ha SG, Liu FT, Rao SP, Sriramarao P
Frontiers in pharmacology 2013;4:37
Frontiers in pharmacology 2013;4:37
Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils.
Doyle AD, Jacobsen EA, Ochkur SI, Willetts L, Shim K, Neely J, Kloeber J, Lesuer WE, Pero RS, Lacy P, Moqbel R, Lee NA, Lee JJ
Journal of leukocyte biology 2013 Jul;94(1):17-24
Journal of leukocyte biology 2013 Jul;94(1):17-24
Inflammation triggers emergency granulopoiesis through a density-dependent feedback mechanism.
Cain DW, Snowden PB, Sempowski GD, Kelsoe G
PloS one 2011;6(5):e19957
PloS one 2011;6(5):e19957
SIRPĪ±/CD172a regulates eosinophil homeostasis.
Verjan Garcia N, Umemoto E, Saito Y, Yamasaki M, Hata E, Matozaki T, Murakami M, Jung YJ, Woo SY, Seoh JY, Jang MH, Aozasa K, Miyasaka M
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2268-77
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2268-77
N-Glycans differentially regulate eosinophil and neutrophil recruitment during allergic airway inflammation.
Bahaie NS, Kang BN, Frenzel EM, Hosseinkhani MR, Ge XN, Greenberg Y, Ha SG, Demetriou M, Rao SP, Sriramarao P
The Journal of biological chemistry 2011 Nov 4;286(44):38231-41
The Journal of biological chemistry 2011 Nov 4;286(44):38231-41
Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice.
Yamada T, Tani Y, Nakanishi H, Taguchi R, Arita M, Arai H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Feb;25(2):561-8
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Feb;25(2):561-8
CD28 and ICOS play complementary non-overlapping roles in the development of Th2 immunity in vivo.
Shilling RA, Clay BS, Tesciuba AG, Berry EL, Lu T, Moore TV, Bandukwala HS, Tong J, Weinstock JV, Flavell RA, Horan T, Yoshinaga SK, Welcher AA, Cannon JL, Sperling AI
Cellular immunology 2009;259(2):177-84
Cellular immunology 2009;259(2):177-84
Intravascular inactivation of CCR5 by n-Nonanoyl-CC chemokine ligand 14 and inhibition of allergic airway inflammation.
Gupta S, Fuchs B, Schulz-Maronde S, Heitland A, Escher SE, Mack M, Tillmann HC, Braun A, Forssmann WG, Elsner J, Forssmann U
Journal of leukocyte biology 2008 Mar;83(3):765-73
Journal of leukocyte biology 2008 Mar;83(3):765-73
CD43 regulates Th2 differentiation and inflammation.
Cannon JL, Collins A, Mody PD, Balachandran D, Henriksen KJ, Smith CE, Tong J, Clay BS, Miller SD, Sperling AI
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7385-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7385-93
Coexpression of IL-5 and eotaxin-2 in mice creates an eosinophil-dependent model of respiratory inflammation with characteristics of severe asthma.
Ochkur SI, Jacobsen EA, Protheroe CA, Biechele TL, Pero RS, McGarry MP, Wang H, O'Neill KR, Colbert DC, Colby TV, Shen H, Blackburn MR, Irvin CC, Lee JJ, Lee NA
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):7879-89
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):7879-89
A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation.
Fulkerson PC, Fischetti CA, McBride ML, Hassman LM, Hogan SP, Rothenberg ME
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 31;103(44):16418-23
Proceedings of the National Academy of Sciences of the United States of America 2006 Oct 31;103(44):16418-23
CD48 is an allergen and IL-3-induced activation molecule on eosinophils.
Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):77-83
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):77-83
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB729F) and Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A). Quadrant markers were set based on control antibody staining (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.
