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Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB155-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCR3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P51677- Isotype
- IgG
- Antibody clone number
- 61828
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Evidence for chemokine synergy during neutrophil migration in ARDS.
Expression and prognostic significance of CCL11/CCR3 in glioblastoma.
Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells.
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
Membrane-bound eotaxin-3 mediates eosinophil transepithelial migration in IL-4-stimulated epithelial cells.
The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts.
Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion.
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells.
Williams AE, José RJ, Mercer PF, Brealey D, Parekh D, Thickett DR, O'Kane C, McAuley DF, Chambers RC
Thorax 2017 Jan;72(1):66-73
Thorax 2017 Jan;72(1):66-73
Expression and prognostic significance of CCL11/CCR3 in glioblastoma.
Tian M, Chen L, Ma L, Wang D, Shao B, Wu J, Wu H, Jin Y
Oncotarget 2016 May 31;7(22):32617-27
Oncotarget 2016 May 31;7(22):32617-27
Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells.
Huang G, Tao L, Shen S, Chen L
Scientific reports 2016 Jun 2;6:27152
Scientific reports 2016 Jun 2;6:27152
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature 2011 Jul 13;475(7355):226-30
Nature 2011 Jul 13;475(7355):226-30
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
International journal of cancer 2010 Jul 15;127(2):332-44
International journal of cancer 2010 Jul 15;127(2):332-44
Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8053-67
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8053-67
The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
Ponte AL, Marais E, Gallay N, Langonné A, Delorme B, Hérault O, Charbord P, Domenech J
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
Stem cells (Dayton, Ohio) 2007 Jul;25(7):1737-45
Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C
Journal of immunology (Baltimore, Md. : 1950) 2006 Sep 1;177(5):3344-54
Journal of immunology (Baltimore, Md. : 1950) 2006 Sep 1;177(5):3344-54
Membrane-bound eotaxin-3 mediates eosinophil transepithelial migration in IL-4-stimulated epithelial cells.
Yuan Q, Campanella GS, Colvin RA, Hamilos DL, Jones KJ, Mathew A, Means TK, Luster AD
European journal of immunology 2006 Oct;36(10):2700-14
European journal of immunology 2006 Oct;36(10):2700-14
The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts.
Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N
The Journal of allergy and clinical immunology 2006 Jan;117(1):103-10
The Journal of allergy and clinical immunology 2006 Jan;117(1):103-10
Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion.
Spencer LA, Melo RC, Perez SA, Bafford SP, Dvorak AM, Weller PF
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 28;103(9):3333-8
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 28;103(9):3333-8
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
Blood 2004 Apr 1;103(7):2806-8
Blood 2004 Apr 1;103(7):2806-8
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
Journal of leukocyte biology 2003 May;73(5):584-90
Journal of leukocyte biology 2003 May;73(5):584-90
Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells.
Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Härtlein M, Kron M, Yang D, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ
The Journal of experimental medicine 2002 Sep 16;196(6):781-91
The Journal of experimental medicine 2002 Sep 16;196(6):781-91
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCR3 in Human Lymph Node. CCR3 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS018) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCR3 Antibody. Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse proB cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant CCL11/Eotaxin (0.333 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155). The ND50 is typically 0.05-0.25 µg/mL.
