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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10705 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10705, RRID:AB_11189540
- Product name
- JAK3 monoclonal antibody, clone 5H2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant JAK3.
- Isotype
- IgG
- Antibody clone number
- 5H2
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) against Jurkat cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of HeLa (upper) and HepG2 (bottom) cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (green) . Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using JAK3 monoclonal antibody, clone 5H2 (Cat # MAB10705) (blue) and negative control (red) .
- Validation comment
- Flow Cytometry
