MA5-15561

antibody from Invitrogen Antibodies

Targeting: JAK3 JAK-3, JAK3_HUMAN, JAKL, L-JAK, LJAK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15561

Provider

Invitrogen Antibodies

Product name

JAK3 Monoclonal Antibody (5H2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

5H2

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Selective Inhibition of Janus Kinase 3 Has No Impact on Infarct Size or Neurobehavioral Outcomes in Permanent Ischemic Stroke in Mice.

DeMars KM, Pacheco SC, Yang C, Siwarski DM, Candelario-Jalil E
Frontiers in neurology 2017;8:363

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of JAK3 using JAK3 monoclonal antibody (Product # MA5-15561) in Jurkat cell lysate (1).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of 3T3-L1 cells using JAK3 monoclonal antibody (Product # MA5-15561) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa (left) and HepG2 (right) cells using JAK3 monoclonal antibody (Product # MA5-15561) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of 3T3-L1 cells using JAK3 monoclonal antibody (Product # MA5-15561) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using JAK3 monoclonal antibody (Product # MA5-15561) (blue) and negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL