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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00001385-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001385-M01, RRID:AB_425380
- Product name
- CREB1 monoclonal antibody (M01), clone 3F1-1B2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CREB1.
- Antigen sequence
MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIAT
LAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVI
QAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTI
AESEDSQESVDSVTDSQKRREILSRRPSYRKILND
LSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIY
QTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMT
NAAATQPGTTILQYAQTTDGQQILVPSNHVVVQAA
SGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAE
EAARKREVRLMKNREAARECRRKKKEYVKCLENRV
AVLENQNKTLIEELKALKDLYCHKSD- Isotype
- IgG
- Antibody clone number
- 3F1-1B2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Distinct pathways leading to TDP-43-induced cellular dysfunctions.
Yamashita M, Nonaka T, Hirai S, Miwa A, Okado H, Arai T, Hosokawa M, Akiyama H, Hasegawa M
Human molecular genetics 2014 Aug 15;23(16):4345-56
Human molecular genetics 2014 Aug 15;23(16):4345-56
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CREB1 expression in transfected 293T cell line by CREB1 monoclonal antibody (M01), clone 3F1-1B2.Lane 1: CREB1 transfected lysate(37 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CREB1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CREB1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CREB1 on formalin-fixed paraffin-embedded human lymph node tissue. [antibody concentration 1 ~ 10 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
