PAB18121

antibody from Abnova Corporation

Targeting: CCNB1 CCNB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB18121

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB18121, RRID:AB_10713030

Product name

CCNB1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of CCNB1.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Phosphorylation of the cyclin b1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx.

Walsh S, Margolis SS, Kornbluth S
Molecular cancer research : MCR 2003 Feb;1(4):280-9

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Combinatorial control of cyclin B1 nuclear trafficking through phosphorylation at multiple sites.

Yang J, Song H, Walsh S, Bardes ES, Kornbluth S
The Journal of biological chemistry 2001 Feb 2;276(5):3604-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of extracts from HeLa cells, treated with serum (10 %, 15 min), using CCNB1 polyclonal antibody (Cat # PAB18121).Peptide "+" means "peptide blocking".

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells, treated with Forskolin (40 nM, 30 mins), using CCNB1 polyclonal antibody (Cat # PAB18121).Peptide "+" means "peptide blocking".

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CCNB1 polyclonal antibody (Cat # PAB18121).Peptide "+" means "peptide blocking".

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)