Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 647902 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#647902, RRID:AB_2244210
- Product name
- Purified anti-Cyclin B1
- Antibody type
- Monoclonal
- Antigen
- Hamster Cyclin B1
- Description
- Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48 kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. The V152 monoclonal antibody recognizes human and mouse Cyclin B1 and has been shown to be useful for Western blotting. and immunohistochemistry (paraffin embedded tissue sections) of human and mouse cyclin B1.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4 °C.
Submitted references Myosin phosphatase-targeting subunit 1 controls chromatid segregation.
Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero.
Matsumura F, Yamakita Y, Yamashiro S
The Journal of biological chemistry 2011 Mar 25;286(12):10825-33
The Journal of biological chemistry 2011 Mar 25;286(12):10825-33
Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero.
Brandeis M, Rosewell I, Carrington M, Crompton T, Jacobs MA, Kirk J, Gannon J, Hunt T
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 14;95(8):4344-9
Proceedings of the National Academy of Sciences of the United States of America 1998 Apr 14;95(8):4344-9
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from Hela (lane 1) and NIH/3T3 (lane 2) cells, using anti-Cyclin B1, V152.
- Conjugate
- Unconjugated