MAB8302

antibody from Abnova Corporation

Targeting: ELK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8302

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB8302, RRID:AB_10616334

Product name

ELK1 monoclonal antibody, clone 3H6D12, 4H9C8,4H9F1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant ELK1.

Isotype

IgG

Antibody clone number

3H6D12, 4H9C8,4H9F1

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

PKCalpha expression regulated by Elk-1 and MZF-1 in human HCC cells.

Hsieh YH, Wu TT, Tsai JH, Huang CY, Hsieh YS, Liu JY
Biochemical and biophysical research communications 2006 Jan 6;339(1):217-25

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Activation of ternary complex factor Elk-1 by stress-activated protein kinases.

Gille H, Strahl T, Shaw PE
Current biology : CB 1995 Oct 1;5(10):1191-200

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ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation.

Gille H, Kortenjann M, Thomae O, Moomaw C, Slaughter C, Cobb MH, Shaw PE
The EMBO journal 1995 Mar 1;14(5):951-62

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis using ELK1 monoclonal antibody, clone 3H6D12, 4H9C8,4H9F1 (Cat # MAB8302) against truncated recombinant ELK1 (Lane 1) and K-562 cell lysate (Lane 2).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoistochemical analysis of paraffin-embedded human brain tumor tissue showing nuclear/cytoplasmic localization using ELK1 with DAB staining.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)