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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-D34 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-D34, RRID:AB_11181290
- Product name
- Anti-Elk-1 pS383 (Rabbit) Antibody - 600-401-D34
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Anti-Elk-1 pS383 (Rabbit) Antibody. Lane 1: recombinant Elk-1 non-phospho. Lane 2: recombinant Elk-1 phospho. Load: 10 µg per lane. Primary antibody: Elk-1 pS383 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~46kDa/~46kDa for Elk-1 phosphorylated at Ser383. Other band(s): none.
- Validation comment
- Western Blot
