NB600-1182
antibody from Novus Biologicals
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB600-1182 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1182, RRID:AB_10001556
- Product name
- Mouse Monoclonal EpCAM/TROP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Shape Engineering Boosts Magnetic Mesoporous Silica Nanoparticle-Based Isolation and Detection of Circulating Tumor Cells.
Both human and mouse mesenchymal stem cells promote breast cancer metastasis.
Type 1 insulin-like growth factor receptor translocates to the nucleus of human tumor cells.
Detection of circulating tumour cells in peripheral blood with an automated scanning fluorescence microscope.
Targeted killing of colorectal cancer cell lines by a humanised IgG1 monoclonal antibody that binds to membrane-bound carcinoembryonic antigen.
FGF-1 and FGF-2 modulate the E-cadherin/catenin system in pancreatic adenocarcinoma cell lines.
Vicia faba agglutinin, the lectin present in broad beans, stimulates differentiation of undifferentiated colon cancer cells.
Vicia faba agglutinin, the lectin present in broad beans, stimulates differentiation of undifferentiated colon cancer cells.
AUA1: new immunocytochemical marker for detecting epithelial cells in body cavity fluids.
AUA1: new immunocytochemical marker for detecting epithelial cells in body cavity fluids.
Immunohistochemical and immunocytochemical study of bladder carcinomas using the epithelium-specific, tumour-associated monoclonal antibodies HMFG1 and AUA1.
Chang ZM, Wang Z, Shao D, Yue J, Xing H, Li L, Ge M, Li M, Yan H, Hu H, Xu Q, Dong WF
ACS applied materials & interfaces 2018 Apr 4;10(13):10656-10663
ACS applied materials & interfaces 2018 Apr 4;10(13):10656-10663
Both human and mouse mesenchymal stem cells promote breast cancer metastasis.
Albarenque SM, Zwacka RM, Mohr A
Stem cell research 2011 Sep;7(2):163-71
Stem cell research 2011 Sep;7(2):163-71
Type 1 insulin-like growth factor receptor translocates to the nucleus of human tumor cells.
Aleksic T, Chitnis MM, Perestenko OV, Gao S, Thomas PH, Turner GD, Protheroe AS, Howarth M, Macaulay VM
Cancer research 2010 Aug 15;70(16):6412-9
Cancer research 2010 Aug 15;70(16):6412-9
Detection of circulating tumour cells in peripheral blood with an automated scanning fluorescence microscope.
Ntouroupi TG, Ashraf SQ, McGregor SB, Turney BW, Seppo A, Kim Y, Wang X, Kilpatrick MW, Tsipouras P, Tafas T, Bodmer WF
British journal of cancer 2008 Sep 2;99(5):789-95
British journal of cancer 2008 Sep 2;99(5):789-95
Targeted killing of colorectal cancer cell lines by a humanised IgG1 monoclonal antibody that binds to membrane-bound carcinoembryonic antigen.
Conaghan P, Ashraf S, Tytherleigh M, Wilding J, Tchilian E, Bicknell D, Mortensen NJ, Bodmer W
British journal of cancer 2008 Apr 8;98(7):1217-25
British journal of cancer 2008 Apr 8;98(7):1217-25
FGF-1 and FGF-2 modulate the E-cadherin/catenin system in pancreatic adenocarcinoma cell lines.
El-Hariry I, Pignatelli M, Lemoine NR
British journal of cancer 2001 Jun 15;84(12):1656-63
British journal of cancer 2001 Jun 15;84(12):1656-63
Vicia faba agglutinin, the lectin present in broad beans, stimulates differentiation of undifferentiated colon cancer cells.
Jordinson M, El-Hariry I, Calnan D, Calam J, Pignatelli M
Gut 1999 May;44(5):709-14
Gut 1999 May;44(5):709-14
Vicia faba agglutinin, the lectin present in broad beans, stimulates differentiation of undifferentiated colon cancer cells.
Jordinson M, El-Hariry I, Calnan D, Calam J, Pignatelli M
Gut 1999 May;44(5):709-14
Gut 1999 May;44(5):709-14
AUA1: new immunocytochemical marker for detecting epithelial cells in body cavity fluids.
Kocjan G, Sweeney E, Miller KD, Bobrow L
Journal of clinical pathology 1992 Apr;45(4):358-9
Journal of clinical pathology 1992 Apr;45(4):358-9
AUA1: new immunocytochemical marker for detecting epithelial cells in body cavity fluids.
Kocjan G, Sweeney E, Miller KD, Bobrow L
Journal of clinical pathology 1992 Apr;45(4):358-9
Journal of clinical pathology 1992 Apr;45(4):358-9
Immunohistochemical and immunocytochemical study of bladder carcinomas using the epithelium-specific, tumour-associated monoclonal antibodies HMFG1 and AUA1.
Anagnostaki E, Skarlos D, Tamvakis N, Psaropoulou P, Blana E, Bamias A, Legaki S, Aravantinos G, Deliveliotis C, Dimopoulos K
The British journal of cancer. Supplement 1990 Jul;10:52-6
The British journal of cancer. Supplement 1990 Jul;10:52-6
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of A431 lysate (A) and Hek293 lysate (B) using EpCAM/CD326 antibody (AUA1) at 2 ug/ml. Non-reduced lysate was used in each lane.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Detection of EpCAM/CD326 in two Human mammary tumor cell lines, MDA-MB-231 (mesenchyme-like) and MCF-7 (epithelium-like). Dilution: 1:500 in PBS with 5% BSA. Secondary Ab: anti-Mouse IgG 1:5,000. This image was submitted via customer Review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This EpCAM antibody generated an expected immunostaining of EpCAM (CD326) protein in the membranes of the cancer cells and the inter-cellular spaces. The tumor stroma and the stromal cells did not show EpCAM immunopositivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue with 1:500 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The staining was primarily localized to the membranes and inter-cellular spaces of the cancer cells. Staining intensity for 1:500 dilution was lower than what was seen in sections tested with 1:100 dilution. Staining was performed by Histowiz.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HeLa (B) cells using EpCAM/CD326 antibody (blue) and matched Isotype control (orange). An antibody concentration of 1 ug/1x10^6 cells was used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Using the PE direct conjugate A cell surface stain was performed on HT-29 cells with EpCAM (AUA1) antibody NB600-1182PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.