AF960
antibody from R&D Systems
Targeting: EPCAM
17-1A, 323/A3, CD326, CO-17A, EGP-2, EGP34, EGP40, Ep-CAM, ESA, GA733-2, HEA125, KS1/4, KSA, Ly74, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AF960 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EpCAM/TROP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human EpCAM/TROP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TROP-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
CAA32870
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system.
HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone.
Fast and selective cell isolation from blood sample by microfiber fabric system with vacuum aspiration.
EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells That Promote Epithelial-to-Mesenchymal Transition.
Stromal gene expression defines poor-prognosis subtypes in colorectal cancer.
Garnier D, Li R, Delbos F, Fourrier A, Collet C, Guguen-Guillouzo C, Chesné C, Nguyen TH
Scientific reports 2018 May 29;8(1):8222
Scientific reports 2018 May 29;8(1):8222
HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone.
Day KC, Lorenzatti Hiles G, Kozminsky M, Dawsey SJ, Paul A, Broses LJ, Shah R, Kunja LP, Hall C, Palanisamy N, Daignault-Newton S, El-Sawy L, Wilson SJ, Chou A, Ignatoski KW, Keller E, Thomas D, Nagrath S, Morgan T, Day ML
Cancer research 2017 Jan 1;77(1):74-85
Cancer research 2017 Jan 1;77(1):74-85
Fast and selective cell isolation from blood sample by microfiber fabric system with vacuum aspiration.
Ueki T, Yoshihara A, Teramura Y, Takai M
Science and technology of advanced materials 2016;17(1):807-815
Science and technology of advanced materials 2016;17(1):807-815
EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells That Promote Epithelial-to-Mesenchymal Transition.
Hsu YT, Osmulski P, Wang Y, Huang YW, Liu L, Ruan J, Jin VX, Kirma NB, Gaczynska ME, Huang TH
Cancer research 2016 Nov 1;76(21):6171-6182
Cancer research 2016 Nov 1;76(21):6171-6182
Stromal gene expression defines poor-prognosis subtypes in colorectal cancer.
Calon A, Lonardo E, Berenguer-Llergo A, Espinet E, Hernando-Momblona X, Iglesias M, Sevillano M, Palomo-Ponce S, Tauriello DV, Byrom D, Cortina C, Morral C, Barceló C, Tosi S, Riera A, Attolini CS, Rossell D, Sancho E, Batlle E
Nature genetics 2015 Apr;47(4):320-9
Nature genetics 2015 Apr;47(4):320-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human EpCAM/TROP-1 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, MCF-7 human breast cancer cell line, and BG01V human embryonic stem cells. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EpCAM/TROP-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EpCAM/TROP-1 in BG01V Human Embryonic Stem Cells. EpCAM/TROP-1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EpCAM/TROP-1 in Human Colon. EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EpCAM/TROP-1 in Human Colon. EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of EpCAM/TROP-1 in HT-29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).