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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00004792-M10 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004792-M10, RRID:AB_11189678
- Product name
- NFKBIA monoclonal antibody (M10), clone 3H4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant NFKBIA.
- Antigen sequence
MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLD
SMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQL
TEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLN
FQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELR
DFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSI
LKATNYNGHTCLHLASIHGYLGIVELLVSLGADVN
AQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNR
VTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQML
PESEDEESYDTESEFTEFTEDELPYDDCVFGGQRL
TL- Isotype
- IgG
- Antibody clone number
- 3H4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NFKBIA monoclonal antibody (M10), clone 3H4. Western Blot analysis of NFKBIA expression in Hela S3 NE.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NFKBIA expression in transfected 293T cell line by NFKBIA monoclonal antibody (M10), clone 3H4.Lane 1: NFKBIA transfected lysate (Predicted MW: 35.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NFKBIA is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NFKBIA on HeLa cell . [antibody concentration 20 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
