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Learn200-301-H81
antibody from Rockland Immunochemicals, Inc.
Targeting: NFKBIA
IkappaBalpha, IKBA, MAD-3, NFKBI
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 200-301-H81 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-H81, RRID:AB_2611288
- Product name
- Anti-IKB? Ser32 Ser36 (MOUSE) Monoclonal Antibody - 200-301-H81
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Mouse Anti-IkBa Ser32 Ser36 antibody. Jurkat cells treated with with 100µg/mL pf ALLN for 30 min. Lane 1: Lysate without 1 nM TNF-a. Lane 2: Lysate with 1 nM TNF-a. Lane 3: Lysate without 1 nM TNF-a. Lane 4: Lysate with 1 nM TNF-a. Load: 30 µg per lane. Primary antibody: IkBa Ser32 Ser36 antibody at 4 µg/mL for overnight at 4°C. Secondary antibody: IRDye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 35.6 kDa for IkBa Ser32 Ser36. Other band(s): both IkB alpha Phoshpo and non-phoshpo.
- Validation comment
- Western Blot
