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- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [2]
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- Product number
- MAB0057 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0057, RRID:AB_1019093
- Product name
- NFKBIA monoclonal antibody, clone 6A920
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant NFKBIA .
- Isotype
- IgG
- Antibody clone number
- 6A920
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references DHMEQ, a novel nuclear factor-kappaB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type 1 cytokines, and blocks maturation of dendritic cells.
Targeting the NF-kappaB signaling pathway in Notch1-induced T-cell leukemia.
BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway.
NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins.
Paeonol attenuates TNBS-induced colitis by inhibiting NF-kappaB and STAT1 transactivation.
Nishioka C, Ikezoe T, Jing Y, Umezawa K, Yokoyama A
Immunology 2008 Jun;124(2):198-205
Immunology 2008 Jun;124(2):198-205
Targeting the NF-kappaB signaling pathway in Notch1-induced T-cell leukemia.
Vilimas T, Mascarenhas J, Palomero T, Mandal M, Buonamici S, Meng F, Thompson B, Spaulding C, Macaroun S, Alegre ML, Kee BL, Ferrando A, Miele L, Aifantis I
Nature medicine 2007 Jan;13(1):70-7
Nature medicine 2007 Jan;13(1):70-7
BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway.
Endo T, Nishio M, Enzler T, Cottam HB, Fukuda T, James DF, Karin M, Kipps TJ
Blood 2007 Jan 15;109(2):703-10
Blood 2007 Jan 15;109(2):703-10
NF-kappaB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins.
Santhi WS, Sebastian P, Varghese BT, Prakash O, Pillai MR
Experimental and molecular pathology 2006 Oct;81(2):123-30
Experimental and molecular pathology 2006 Oct;81(2):123-30
Paeonol attenuates TNBS-induced colitis by inhibiting NF-kappaB and STAT1 transactivation.
Ishiguro K, Ando T, Maeda O, Hasegawa M, Kadomatsu K, Ohmiya N, Niwa Y, Xavier R, Goto H
Toxicology and applied pharmacology 2006 Nov 15;217(1):35-42
Toxicology and applied pharmacology 2006 Nov 15;217(1):35-42
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of NFKBIA. Using NFKBIA monoclonal antibody, clone 6A920 (Cat # MAB0057) at 2 ug/mL in (A) Daudi and (B) NIH/3T3 whole cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Jurkat cells were treated for 30 min with 100 ug/mL ALLN (N-Acetyl-Leu-Leu-Norleucinal; a Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation) followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3). The membranes were blotted with NFKBIA monoclonal antibody, clone 39A1413 (Cat # MAB0056) (lanes 1 & 2) or NFKBIA monoclonal antibody, clone 6A920 (Cat # MAB0057) (that recognizes both non-phospho and phosphorylated forms of IkBa) (lanes 3 & 4) and immunoreactivity was detected by ECL. The data shows that Cat # MAB0056 detects specifically the phosphorylated form of NFKBIA.
