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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- 10-6003 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-IkB alpha Antibody
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant IkB a
lpha protein (amino acids 28-309) w
as used as immunogen for this antib
ody.- Isotype
- IgG
- Antibody clone number
- ABM10F4
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of IkB alpha. Anti-IkB alpha antibody (Clone: ABM10F4) was used at 4 µg/ml on MCF7 and PC3 lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intra cellular flow analysis of IkB alpha in Hela using 0.5 µg/10^6 cells of antibody (Clone: ABM10F4). Green represents isotype control; red represents anti-IkB alpha antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intra cellular flow analysis of IkB alpha in Jurkat using 0.5 µg/10^6 cells of antibody (Clone: ABM10F4). Green represents isotype control; red represents anti-IkB alpha antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
- Protocol
- Protocol
