- MA5-15132 - Invitrogen Antibodies NFKBIA antibody

Submitted references Effects of 3'-isovaleryl-4'-senecioylkhellactone from Peucedanum japonicum Thunberg on PMA-Stimulated Inflammatory Response in A549 Human Lung Epithelial Cells.

Hwang D, Ryu HW, Park JW, Kim JH, Kim DY, Oh JH, Kwon OK, Han SB, Ahn KS
Journal of microbiology and biotechnology 2022 Jan 28;32(1):81-90

Clostridium butyricum Helps to Alleviate Inflammation in Weaned Piglets Challenged With Enterotoxigenic Escherichia coli K88.

Li H, Liu X, Shang Z, Qiao J
Frontiers in veterinary science 2021;8:683863

Nobiletin exerts anti-diabetic and anti-inflammatory effects in an in vitro human model and in vivo murine model of gestational diabetes.

Nguyen-Ngo C, Salomon C, Quak S, Lai A, Willcox JC, Lappas M
Clinical science (London, England : 1979) 2020 Mar 27;134(6):571-592

The short-chain fatty acids butyrate and propionate protect against inflammation-induced activation of mediators involved in active labor: implications for preterm birth.

Moylan HEC, Nguyen-Ngo C, Lim R, Lappas M
Molecular human reproduction 2020 Jun 1;26(6):452-468

Legionella pneumophila regulates the activity of UBE2N by deamidase-mediated deubiquitination.

Gan N, Guan H, Huang Y, Yu T, Fu J, Nakayasu ES, Puvar K, Das C, Wang D, Ouyang S, Luo ZQ
The EMBO journal 2020 Feb 17;39(4):e102806

Short-Term Microgravity Influences Cell Adhesion in Human Breast Cancer Cells.

Nassef MZ, Kopp S, Melnik D, Corydon TJ, Sahana J, Krüger M, Wehland M, Bauer TJ, Liemersdorf C, Hemmersbach R, Infanger M, Grimm D
International journal of molecular sciences 2019 Nov 15;20(22)

Pistacia weinmannifolia root exerts a protective role in ovalbumin‑induced lung inflammation in a mouse allergic asthma model.

Lee JW, Min JH, Kim MG, Kim SM, Kwon OK, Oh TK, Lee JK, Kim TY, Lee SW, Choi S, Li WY, Ryu HW, Ahn KS, Oh SR
International journal of molecular medicine 2019 Dec;44(6):2171-2180

Inhibition of NF-κB activity by the porcine epidemic diarrhea virus nonstructural protein 1 for innate immune evasion.

Zhang Q, Ma J, Yoo D
Virology 2017 Oct;510:111-126

An autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells.

Jia D, Li L, Andrew S, Allan D, Li X, Lee J, Ji G, Yao Z, Gadde S, Figeys D, Wang L
Cell death & disease 2017 Jul 13;8(7):e2932

Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo.

Jia D, Tan Y, Liu H, Ooi S, Li L, Wright K, Bennett S, Addison CL, Wang L
Oncotarget 2016 Jan 5;7(1):771-85

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockout of IkB alpha was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR993809_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of IkB alpha was performed by loading 30 µg of HeLa Wild Type (Lane 1), HeLa Cas9 (Lane 2) andHeLa IkB alpha KO (Lane 3) modified whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with an IkB alpha Monoclonal Antibody (T.937.7) (Product # MA5-15132, 1:1,000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to IkB alpha.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of IkB alpha using a monoclonal antibody (Product # MA5-15132).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extract (30 µg lysate) of HeLa (Lane 1), HeLa treated with TNF alpha (20 ng/mL, 10 min) (Lane 2), NIH/3T3 (Lane 3), NIH/3T3 treated with TNF alpha (20 ng/mL, 10 min) (Lane 4), THP-1 (Lane 5), THP-1 treated with TPA (80 nM for 24 hours) (Lane 6), THP-1 treated with TPA (80 nM for 24 hours) followed by LPS (1 µg/mL for 30 minutes) (Lane 7) and THP-1 treated with TPA (80 nM for 24 hours) followed by LPS (1 µg/mL for 6 hours) (Lane 8). The blot was probed with Anti-IkB alpha Monoclonal Antibody (6A920) (Product # MA5-15132, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 36 kDa band corresponding to IkB alpha was observed in HeLa, NIH/3T3 and THP-1 and was altered upon cell treatment.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of I-kappa-B-alpha in HeLa cells using an I-kappa-B-alpha monoclonal antibody (Product # MA5-15132) (red). DNA is labeled using a fluorescent blue dye.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of I-kappa-B-alpha in paraffin-embedded human lung carcinoma using a I-kappa-B-alpha monoclonal antibody (Product # MA5-15132).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometric analysis of I-kappa-B-alpha in HeLa cells using a I-kappa-B-alpha monoclonal antibody (Product # MA5-15132) (blue) compared to a nonspecifc negative control antibody (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Effect of CB on the activation of NF-kappaB p65 and IkappaBalpha in ETEC K88-infected piglets. Pigs were challenged with or without ETEC (1 x 10 9 CFU/kg BW). At 3, 6, and 12 h post inflammatory challenge, intestinal mucosa samples were collected to examine the expression of p65, p-p65, IkappaBalpha, and p-IkappaBalpha by Western blotting. NC, piglets were fed the daily diet and received oral administration of sterile physiological saline; PC, piglets were fed the daily diet and received oral challenge with ETEC K88; PC + CB, piglets were fed the CB-supplemented diet and received oral challenge with ETEC K88.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 7 Effect of PWRE on OVA-induced activation of NF-kappaB p65 and IkappaBalpha molecules in the lungs. (A) The levels of NF-kappaB and IkappaB activation in the lung tissues were assessed via western blot analysis. (B) Quantitative analysis of p-NF-kappaB p65 and (C) p-IkappaB was performed by densitometric analysis. # P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 9 Effect of PW on LPS-stimulated activation of NF-kappaB p65 and IkappaBalpha in RAW264.7 cells. (A) The levels of NF-kappaB and IkappaB activation were evaluated via western blot analysis. (B) Quantitative analysis of p-NF-kappaB p65 and p-IkappaB was performed by densitometric analysis. # P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7 Inhibition of p65 nuclear translocation by nsp1 and inhibition of IkappaBalpha phosphorylation. (A) and (B), PEDV nsp1 blocks p65 nuclear translocation. HeLa cells were co-transfected with pXJ41 (empty plasmid), nsp1, N, and PRRSV nsp1alpha for 24 h followed by stimulation with TNFalpha (15 ng/ml) for 20 min. Cells were then subjected to immunostaining. PRRSV nsp1alpha is known to inhibit p65 nuclear translocation and was included as a positive control. White arrows indicates p65 in mock-transfected cells, and yellow arrows indicate p65 in gene-transfected cells (panel A). The percentages of cells showing p65 activation were calculated by examining 200 gene-transfected cells in three independent experiments (panel B). Asterisks indicate statistical significance calculated by the Student's t -test. *, P

MA5-15132

Antibody data