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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- PAB12635 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12635, RRID:AB_10552051
- Product name
- MAP2K1/MAP2K2 (phospho S218/S222) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide corresponding to residues surrounding S218/S222 of human MAP2K1/MAP2K2.
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
SMANS- Vial size
- 100 µg
- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Inhibition of the Raf-MEK1/2-ERK1/2 signaling pathway, Bcl-xL down-regulation, and chemosensitization of non-Hodgkin's lymphoma B cells by Rituximab.
Jazirehi AR, Vega MI, Chatterjee D, Goodglick L, Bonavida B
Cancer research 2004 Oct 1;64(19):7117-26
Cancer research 2004 Oct 1;64(19):7117-26
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The whole cell lysates derived from Starved NIH/3T3 were immunoblotted by MAP2K1/MAP2K2 (phospho S218/222) polyclonal antibody (Cat # PAB12635) at 1 : 1000 (lane 1). The lane 2 was a negative control.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot Blot : 1 ug peptide was blot onto NC membrane.A : Phospho-MAP2K1/MAP2K2 (pS218/222).B : MAP2K1/MAP2K2 (Nonphospho).C : Non-related phospho-specific phosphopeptide.Followed were blotted at a 1 : 1000 dilution by :1. MAP2K1/MAP2K2 (phospho S218/222) polyclonal antibody (Cat # PAB12635).2. MAP2K1/MAP2K2 polyclonal antibody (Cat # PAB12707).
- Validation comment
- Dot Blot (Peptide)
