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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX102391 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102391, RRID:AB_1950857
- Product name
- MEK1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71.
Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction.
Tang WF, Huang RT, Chien KY, Tang P, Horng JT
Journal of proteome research 2019 Jan 4;18(1):449-460
Journal of proteome research 2019 Jan 4;18(1):449-460
Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction.
Wang TH, Hsueh C, Chen CC, Li WS, Yeh CT, Lian JH, Chang JL, Chen CY
International journal of molecular sciences 2018 Sep 10;19(9)
International journal of molecular sciences 2018 Sep 10;19(9)
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE GTX102391 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX102391 diluted at 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membranes were blotted with MEK1 antibody (GTX102391) diluted at 1:1000 and competitor's antibody (SC-219) diluted by 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using MEK1(GTX102391) antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MEK1 antibody detects MEK1 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. MEK1 antibody (GTX102391) diluted at 1:500.
