GTX121942

antibody from GeneTex

Targeting: MAP2K1 MAPKK1, MEK1, PRKMK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX121942

Provider

GeneTex

Proper citation

GeneTex Cat#GTX121942, RRID:AB_10724117

Product name

MEK1 + MEK2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

IL-1β-Induced Mesenchymal Stem Cell Migration Involves MLCK Activation via PKC Signaling.

Lin CY, Zu CH, Yang CC, Tsai PJ, Shyu JF, Chen CP, Weng ZC, Chen TH, Wang HS
Cell transplantation 2015;24(10):2011-28

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miR-524-5p suppresses the growth of oncogenic BRAF melanoma by targeting BRAF and ERK2.

Liu SM, Lu J, Lee HC, Chung FH, Ma N
Oncotarget 2014 Oct 15;5(19):9444-59

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 μg of whole cell lysate) A: 293T 10% SDS PAGE GTX121942 diluted at 1:3000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 μg of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE GTX121942 diluted at 1:2000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

MEK1+2 antibody detects MEK1+2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MEK1+2 protein stained by MEK1+2 antibody (GTX121942) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

MEK1+2 antibody detects MEK1+2 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. MEK1+2 antibody (GTX121942) diluted at 1:500.