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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PAB0529 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0529, RRID:AB_982684
- Product name
- MAP2K1 (phospho S218/S222) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular cross-talk between MEK1/2 and mTOR signaling during recovery of 293 cells from hypertonic stress.
MEK1 and MEK2, different regulators of the G1/S transition.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Naegele S, Morley SJ
The Journal of biological chemistry 2004 Oct 29;279(44):46023-34
The Journal of biological chemistry 2004 Oct 29;279(44):46023-34
MEK1 and MEK2, different regulators of the G1/S transition.
Ussar S, Voss T
The Journal of biological chemistry 2004 Oct 15;279(42):43861-9
The Journal of biological chemistry 2004 Oct 15;279(42):43861-9
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAP2K1 (phospho S217/221) polyclonal antibody (Cat # PAB0529) in Ramos cell lysate (35 ug/lane). Phospho-MAP2K1 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Validation comment
- Western Blot (Cell lysate)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAP2K1 (phospho S217/221) polyclonal antibody (Cat # PAB0529) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of MAP2K1 (phospho S217/S221) polyclonal antibody (Cat # PAB0529) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
