M00292-1

antibody from Boster Biological Technology

Targeting: MAP2K1 MAPKK1, MEK1, PRKMK1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00292-1

Provider

Boster Biological Technology

Product name

Anti-MEK1/2 MAP2K1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for MEK1/MAP2K1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat MEK1/MAP2K1 information: Molecular Weight: 43439 MW; Subcellular Localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis; Tissue Specificity: Widely expressed, with extremely low levels in brain.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

BOA-13

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human kidney, using MEK1/2 Antibody(M00292-1)MAP2K1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAP2K1 Antibody (M00292-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image