Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB3174 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3174, RRID:AB_1577140
- Product name
- MAP2K2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAP2K2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2.
p38 Mitogen-activated protein kinase pathway suppresses cell survival by inducing dephosphorylation of mitogen-activated protein/extracellular signal-regulated kinase kinase1,2.
Phosphatidylinositol 3-kinase and mek1/2 are necessary for insulin-like growth factor-I-induced vascular endothelial growth factor synthesis in prostate epithelial cells: a role for hypoxia-inducible factor-1?
Tran H, Maurer F, Nagamine Y
Molecular and cellular biology 2003 Oct;23(20):7177-88
Molecular and cellular biology 2003 Oct;23(20):7177-88
p38 Mitogen-activated protein kinase pathway suppresses cell survival by inducing dephosphorylation of mitogen-activated protein/extracellular signal-regulated kinase kinase1,2.
Li SP, Junttila MR, Han J, Kähäri VM, Westermarck J
Cancer research 2003 Jul 1;63(13):3473-7
Cancer research 2003 Jul 1;63(13):3473-7
Phosphatidylinositol 3-kinase and mek1/2 are necessary for insulin-like growth factor-I-induced vascular endothelial growth factor synthesis in prostate epithelial cells: a role for hypoxia-inducible factor-1?
Burroughs KD, Oh J, Barrett JC, DiAugustine RP
Molecular cancer research : MCR 2003 Feb;1(4):312-22
Molecular cancer research : MCR 2003 Feb;1(4):312-22
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The MAP2K2 polyclonal antibody (Cat # PAB3174) is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAP2K2 polyclonal antibody (Cat # PAB3174) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)