Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C745381 - Provider product page
- Provider
- LSBio
- Product name
- MAP2K2 / MKK2 / MEK2 Antibody (N-Terminus) LS-C745381
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Anti-MEK2 N-term Antibody. Lane 1: MEK1 rec lysate. Lane 2: MEK2 rec lysate. Lane 3: Mouse Brain Whole cell lysate. Lane 4: HEK293T Whole cell lysate. Load: 10ug. Primary Antibody: Anti-MEK2 at 1µg/mL overnight at 4°C. Secondary Antibody: Goat Anti-Rabbit Peroxidase Conjugated Antibody at 1:40,000 for 30 min at RT. Blocking: BlockOut Universal Blocking blocking buffer MB-073. Predicted MW: 45kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry with anti-MEK2 (N-Term) antibody showing positive staining in human glioblastoma tissue at 20x and 40x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval and then incubated with rabbit anti-MEK2 (N-Term) antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3?-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.