A700-079
antibody from Invitrogen Antibodies
Targeting: MAP2K2
MEK2, PRKMK2
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- A700-079 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEK2 Recombinant Rabbit Monoclonal Antibody (BLR079G)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR079G
- Vial size
- 100 µL
- Concentration
- 100 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse MEK2 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, HEK293T, A-549, Jurkat, RKO, U2OS, LNCaP, HeLa, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (Product # A700-079 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH (Product # A300-639A).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MEK2 in FFPE KG-1 cells by ICC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (Product # A700-079 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MEK2 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (BLR079G) (A700-079 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MEK2 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (Product # A700-079 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MEK2 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-MEK2 recombinant monoclonal [BLR079G] (Product #A700-079; lot 1) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human MEK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (Product # A700-079 lot 1) used at 20 µL/mg lysate. For blotting immunoprecipitated MEK2, A700-079 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.