A700-079

antibody from Invitrogen Antibodies

Targeting: MAP2K2 MEK2, PRKMK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-079

Provider

Invitrogen Antibodies

Product name

MEK2 Recombinant Rabbit Monoclonal Antibody (BLR079G)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR079G

Vial size

100 µL

Concentration

100 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse MEK2 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, HEK293T, A-549, Jurkat, RKO, U2OS, LNCaP, HeLa, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (Product # A700-079 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH (Product # A300-639A).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MEK2 in FFPE KG-1 cells by ICC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (Product # A700-079 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MEK2 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (BLR079G) (A700-079 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MEK2 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (Product # A700-079 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MEK2 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-MEK2 recombinant monoclonal [BLR079G] (Product #A700-079; lot 1) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human MEK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody [BLR079G] (Product # A700-079 lot 1) used at 20 µL/mg lysate. For blotting immunoprecipitated MEK2, A700-079 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.