GTX100573

antibody from GeneTex

Targeting: PARP1 ADPRT, ARTD1, PARP, PPOL

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX100573

Provider

GeneTex

Proper citation

GeneTex Cat#GTX100573, RRID:AB_1241155

Product name

PARP antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.

Huang MH, Chou YW, Li MH, Shih TE, Lin SZ, Chuang HM, Chiou TW, Su HL, Harn HJ
Pharmacological research 2019 Jan;139:50-61

---

Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.

Lee CW, Chi MC, Chang TM, Liu JF
Journal of cellular physiology 2019 Aug;234(8):13157-13168

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Pterostilbene modulates the suppression of multidrug resistance protein 1 and triggers autophagic and apoptotic mechanisms in cisplatin-resistant human oral cancer CAR cells via AKT signaling.

Chang HP, Lu CC, Chiang JH, Tsai FJ, Juan YN, Tsao JW, Chiu HY, Yang JS
International journal of oncology 2018 May;52(5):1504-1514

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Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway.

Lin SC, Chu PY, Liao WT, Wu MY, Tsui KH, Lin LT, Huang CH, Chen LL, Li CJ
Oncology reports 2018 Feb;39(2):703-710

---

Dual targeting delivery of miR-328 by functionalized mesoporous silica nanoparticles for colorectal cancer therapy.

Li Y, Duo Y, Zhai P, He L, Zhong K, Zhang Y, Huang K, Luo J, Zhang H, Yu X
Nanomedicine (London, England) 2018 Aug 7;

---

Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain.

Dominguez G, Maddelein ML, Pucelle M, Nicaise Y, Maurage CA, Duyckaerts C, Cuvillier O, Delisle MB
Acta neuropathologica communications 2018 Apr 3;6(1):25

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Response of Myeloid Leukemia Cells to Luteolin is Modulated by Differentially Expressed Pituitary Tumor-Transforming Gene 1 (PTTG1) Oncoprotein.

Chen PY, Tien HJ, Chen SF, Horng CT, Tang HL, Jung HL, Wu MJ, Yen JH
International journal of molecular sciences 2018 Apr 12;19(4)

---

The microRNA-302b-inhibited insulin-like growth factor-binding protein 2 signaling pathway induces glioma cell apoptosis by targeting nuclear factor IA.

Lee CC, Chen PH, Ho KH, Shih CM, Cheng CH, Lin CW, Cheng KT, Liu AJ, Chen KC
PloS one 2017;12(3):e0173890

---

Repurposing Albendazole: new potential as a chemotherapeutic agent with preferential activity against HPV-negative head and neck squamous cell cancer.

Ghasemi F, Black M, Vizeacoumar F, Pinto N, Ruicci KM, Le CCSH, Lowerison MR, Leong HS, Yoo J, Fung K, MacNeil D, Palma DA, Winquist E, Mymryk JS, Boutros PC, Datti A, Barrett JW, Nichols AC
Oncotarget 2017 Sep 22;8(42):71512-71519

---

Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma.

Li SM, Chen CH, Chen YW, Yen YC, Fang WT, Tsai FY, Chang JL, Shen YY, Huang SF, Chuu CP, Chang IS, Hsiung CA, Jiang SS
Scientific reports 2017 Sep 19;7(1):11893

---

PARP1 controls KLF4-mediated telomerase expression in stem cells and cancer cells.

Hsieh MH, Chen YT, Chen YT, Lee YH, Lu J, Chien CL, Chen HF, Ho HN, Yu CJ, Wang ZQ, Teng SC
Nucleic acids research 2017 Oct 13;45(18):10492-10503

---

Panobinostat sensitizes KRAS-mutant non-small-cell lung cancer to gefitinib by targeting TAZ.

Lee WY, Chen PC, Wu WS, Wu HC, Lan CH, Huang YH, Cheng CH, Chen KC, Lin CW
International journal of cancer 2017 Nov 1;141(9):1921-1931

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DHEA protects mitochondria against dual modes of apoptosis and necroptosis in human granulosa HO23 cells.

Tsui KH, Wang PH, Lin LT, Li CJ
Reproduction (Cambridge, England) 2017 Aug;154(2):101-110

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Oncogenic miR-137 contributes to cisplatin resistance via repressing CASP3 in lung adenocarcinoma.

Su TJ, Ku WH, Chen HY, Hsu YC, Hong QS, Chang GC, Yu SL, Chen JJ
American journal of cancer research 2016;6(6):1317-30

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Photodynamic-therapy Activates Immune Response by disrupting Immunity Homeostasis of Tumor Cells, which Generates Vaccine for Cancer Therapy.

Zheng Y, Yin G, Le V, Zhang A, Chen S, Liang X, Liu J
International journal of biological sciences 2016;12(1):120-32

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The Inhibition of microRNA-128 on IGF-1-Activating mTOR Signaling Involves in Temozolomide-Induced Glioma Cell Apoptotic Death.

Chen PH, Cheng CH, Shih CM, Ho KH, Lin CW, Lee CC, Liu AJ, Chang CK, Chen KC
PloS one 2016;11(11):e0167096

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The Rheumatoid Arthritis Risk Variant CCR6DNP Regulates CCR6 via PARP-1.

Li G, Cunin P, Wu D, Diogo D, Yang Y, Okada Y, Plenge RM, Nigrovic PA
PLoS genetics 2016 Sep;12(9):e1006292

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Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.

Chakraborty A, Tapryal N, Venkova T, Horikoshi N, Pandita RK, Sarker AH, Sarkar PS, Pandita TK, Hazra TK
Nature communications 2016 Oct 5;7:13049

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The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.

Chen PH, Chang CK, Shih CM, Cheng CH, Lin CW, Lee CC, Liu AJ, Ho KH, Chen KC
Neuropharmacology 2016 Nov;110(Pt A):362-375

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An Artificial Reaction Promoter Modulates Mitochondrial Functions via Chemically Promoting Protein Acetylation.

Shindo Y, Komatsu H, Hotta K, Ariga K, Oka K
Scientific reports 2016 Jul 4;6:29224

---

Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.

Xu YM, Wu DD, Zheng W, Yu FY, Yang F, Yao Y, Zhou Y, Ching YP, Lau AT
Oncotarget 2016 Feb 2;7(5):6146-58

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Xanthohumol induces growth inhibition and apoptosis in ca ski human cervical cancer cells.

Yong WK, Abd Malek SN
Evidence-based complementary and alternative medicine : eCAM 2015;2015:921306

---

Inhibition of colon cancer cell growth by nanoemulsion carrying gold nanoparticles and lycopene.

Huang RF, Wei YJ, Inbaraj BS, Chen BH
International journal of nanomedicine 2015;10:2823-46

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Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.

Lee WY, Lee WT, Cheng CH, Chen KC, Chou CM, Chung CH, Sun MS, Cheng HW, Ho MN, Lin CW
Oncotarget 2015 Sep 29;6(29):27580-95

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Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.

Lee WY, Lee WT, Cheng CH, Chen KC, Chou CM, Chung CH, Sun MS, Cheng HW, Ho MN, Lin CW
Oncotarget 2015 Sep 29;6(29):27580-95

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Lovastatin overcomes gefitinib resistance through TNF-α signaling in human cholangiocarcinomas with different LKB1 statuses in vitro and in vivo.

Yang SH, Lin HY, Chang VH, Chen CC, Liu YR, Wang J, Zhang K, Jiang X, Yen Y
Oncotarget 2015 Sep 15;6(27):23857-73

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Autophagy inhibition enhances isorhamnetin‑induced mitochondria‑dependent apoptosis in non‑small cell lung cancer cells.

Ruan Y, Hu K, Chen H
Molecular medicine reports 2015 Oct;12(4):5796-806

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The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion.

Chou SF, Tsai ML, Huang JY, Chang YS, Shih C
PLoS pathogens 2015 Oct;11(10):e1005123

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Three Pathways Assess Anti-Inflammatory Response of Epicatechin with Lipopolysaccharide-Mediated Macrophage RAW264.7 Cells

Yang D, Liu S, Chen Y, Hsu S, Chang Y, Lin J
Journal of Food Biochemistry 2015 June;39(3):334-343

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PGRMC1 contributes to doxorubicin-induced chemoresistance in MES-SA uterine sarcoma.

Lin ST, May EW, Chang JF, Hu RY, Wang LH, Chan HL
Cellular and molecular life sciences : CMLS 2015 Jun;72(12):2395-409

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Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.

Li CJ, Sun LY, Pang CY
Scientific reports 2015 Apr 24;5:9819

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Induction of ROS-independent JNK-activation-mediated apoptosis by a novel coumarin-derivative, DMAC, in human colon cancer cells.

Lin MH, Cheng CH, Chen KC, Lee WT, Wang YF, Xiao CQ, Lin CW
Chemico-biological interactions 2014 Jul 25;218:42-9

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Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma.

Li KC, Hua KT, Lin YS, Su CY, Ko JY, Hsiao M, Kuo ML, Tan CT
Molecular cancer 2014 Jul 15;13:172

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MicroRNA-302b-inhibited E2F3 transcription factor is related to all trans retinoic acid-induced glioma cell apoptosis.

Chen PH, Shih CM, Chang WC, Cheng CH, Lin CW, Ho KH, Su PC, Chen KC
Journal of neurochemistry 2014 Dec;131(6):731-42

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Anti-proliferative and gene expression actions of resveratrol in breast cancer cells in vitro.

Chin YT, Hsieh MT, Yang SH, Tsai PW, Wang SH, Wang CC, Lee YS, Cheng GY, HuangFu WC, London D, Tang HY, Fu E, Yen Y, Liu LF, Lin HY, Davis PJ
Oncotarget 2014 Dec 30;5(24):12891-907

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Inhibitory effect of litchi (Litchi chinensis Sonn.) flower on lipopolysaccharide-induced expression of proinflammatory mediators in RAW264.7 cells through NF-κB, ERK, and JAK2/STAT3 inactivation.

Yang DJ, Chang YY, Lin HW, Chen YC, Hsu SH, Lin JT
Journal of agricultural and food chemistry 2014 Apr 16;62(15):3458-65

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Biliverdin's regulation of reactive oxygen species signalling leads to potent inhibition of proliferative and angiogenic pathways in head and neck cancer.

Zheng J, Nagda DA, Lajud SA, Kumar S, Mouchli A, Bezpalko O, O'Malley BW Jr, Li D
British journal of cancer 2014 Apr 15;110(8):2116-22

---

Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways.

Li CJ, Tsang SF, Tsai CH, Tsai HY, Chyuan JH, Hsu HY
Evidence-based complementary and alternative medicine : eCAM 2012;2012:261971

---

Subamolide A, a component isolated from Cinnamomum subavenium, induces apoptosis mediated by mitochondria-dependent, p53 and ERK1/2 pathways in human urothelial carcinoma cell line NTUB1.

Liu CH, Chen CY, Huang AM, Li JH
Journal of ethnopharmacology 2011 Sep 1;137(1):503-11

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Interactome study suggests multiple cellular functions of hepatoma-derived growth factor (HDGF).

Zhao J, Yu H, Lin L, Tu J, Cai L, Chen Y, Zhong F, Lin C, He F, Yang P
Journal of proteomics 2011 Dec 21;75(2):588-602

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

39596

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: 293T B: HeLa 7.5% SDS PAGE GTX100573 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PARP1 antibody detects PARP1 protein by western blot analysis.A. 30 ?g HCT116 whole cell lysate/extract (untreated)B. 30 ?g HCT116 whole cell lysate/extract (30 ?M cisplatin treatment for 24hr)7.5% SDS-PAGEPARP1 antibody (GTX100573) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP antibody (GTX100573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membranes were blotted with PARP antibody (GTX100573) diluted at 1:2000 and competitor's antibody (#9542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PARP1(GTX100573) antibody at 1:200 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PARP antibody detects PARP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PARP protein stained by PARP antibody (GTX100573) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 £gm.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PARP antibody detects PARP protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. PARP antibody (GTX100573) diluted at 1:500.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cross-linked ChIP was performed with Raji chromatin extract and 5 £gg of either control rabbit IgG or anti-PARP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to S100A9 promoter.