Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- R31722 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PARP Antibody
- Antibody type
- Polyclonal
- Antigen
- Human partial recombinant protein (AA 670-858) was used as the immunogen for this PARP antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the PARP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) HepG2, 3) COLO-320, 4) Jurkat, 5) rat PC-12, 6) mouse NIH 3T3 and 7) mouse HEPA 1-6 lysate with PARP antibody at 0.5ug/ml. Predicted molecular weight: ~116 kDa (full length), ~89 kDa (C-terminal catalytic domain).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of PARP antibody and A549 cells
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: PARP antibody testing of mouse intestine tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: PARP antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: PARP antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.