- HPA045168 - Atlas Antibodies PARP1 antibody

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Enhanced validation

Submitted by: klas2
Enhanced method: Genetic validation
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Experimental details: Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting PARP1 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting PARP1 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
Sample type: U-2 OS
Primary Ab dilution: 1:180
Conjugate: Horseradish Peroxidase
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:3000
Knockdown/Genetic Approaches Application: Western blot
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: s1097
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Prod no 2: s1098
KD Reagent Page 3: KD Reagent Page 3
KD Reagent Prod no 3: s1099
Antibody Lot Number: R42199
Appendix: 56a72241e4dfb.pdf

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PARP1 antibody. Remaining relative intensity is presented

Supportive validation

Enhanced validation

Submitted by: 55af80e3e0991
Enhanced method: Genetic validation
Main image
Experimental details: Confocal images of immunofluorescently stained human U-2 OS cells.The protein PARP1 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where PARP1 has been downregulated with specific siRNA.
Sample type: U-2 OS cells
Primary Ab dilution: 1:81
Secondary Ab: Secondary Ab
Secondary Ab dilution: 1:800
Knockdown/Genetic Approaches Application: Immunocytochemistry
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: Thermo Fisher Scientific
KD Reagent Prod no: s1097
KD Reagent Prod no 2: s1098
KD Reagent Page 2: KD Reagent Page 2
KD Reagent Page 3: KD Reagent Page 3
KD Reagent Prod no 3: s1099
Antibody Lot Number: R42199
Appendix: 55b73deee1bf8.pdf

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus.
Sample type: HUMAN

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human lymph node and pancreas tissues using HPA045168 antibody. Corresponding PARP1 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human parathyroid gland shows strong nuclear positivity in glandular cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human lymph node shows strong nuclear positivity in lymphoid cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human pancreas shows strong nuclear positivity.
Sample type: HUMAN

HPA045168