Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C154235 - Provider product page
- Provider
- LSBio
- Product name
- PRKCB / PKC-Beta Antibody LS-C154235
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Simian
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-PKC Beta Antibody - Western Blot. Western blot of affinity purified anti-PKC beta antibody shows detection of PKC beta in ~25 ug of U251 whole cell lysate (glioma derived). A 4-12% gel was used for separation. The arrowhead corresponds to 76 kD PKC beta. The membrane was probed with the primary antibody at a 1:1000 dilution in 5% BSA in TTBS at 4C, overnight. Personal Communication, Howard Fine and Svetlana Kotliarova, CCR-NCI, Bethesda, MD.