GTX113252

PRKCB antibody from GeneTex

PKC?, PKCB, PRKCB1, PRKCB2

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Functional assay

Data presented on provider website

Provider product page for GTX113252

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Antibody Data

Product number

GTX113252

Provider

GeneTex

Proper citation

GeneTex Cat#GTX113252, RRID:AB_10729642

Product name

PKC beta antibody

Provider product page

Antibody type

Polyclonal

Reactivity

Human, Rat, Xenopus

Host

Rabbit

References

Antiviral effect of 7-(4-benzimidazole-butoxy)-coumarin on rhabdoviral clearance via Nrf2 activation regulated by PKCα/β phosphorylation.
Liu L, Shen YF, Hu Y, Lu JF
Fish & shellfish immunology 2018 Dec;83:386-396

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Interleukin-1β mediates high glucose induced phenotypic transition in human aortic endothelial cells.
Zhu DD, Tang RN, Lv LL, Wen Y, Liu H, Zhang XL, Ma KL, Liu BC
Cardiovascular diabetology 2016 Mar 5;15:42

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cPKC regulates interphase nuclear size during Xenopus development.
Edens LJ, Levy DL
The Journal of cell biology 2014 Aug 18;206(4):473-83

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX113252 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC beta antibody (GTX113252) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using PKC beta(GTX113252) antibody at 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

PKC beta antibody detects PKC beta protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PKC beta antibody (GTX113252) diluted at 1:500.