Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 44-964G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PKC beta-2 (Thr641) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Demonstration of the essential role of protein kinase C isoforms in hyperglycemia-induced embryonic malformations.
Zhiyong Zhao, Wu YK, Reece EA
Reproductive sciences (Thousand Oaks, Calif.) 2008 Apr;15(4):349-56
Reproductive sciences (Thousand Oaks, Calif.) 2008 Apr;15(4):349-56
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PKC-beta II (pT641) phosphospecific antibody. PMA-treated K652 cell lysates were incubated with anti-PKC-beta II (pT641) after prior incubation with: no peptide (1, 2), the non-phosphopeptide (3), a generic (pT) peptide (4), or, the phosphopeptide immunogen (5). Peptide competition data show antibody phospho-site specificity. Lane 1 was treated with l phosphatase prior to immunoblot. Absence of signal further demonstrates antibody specificity.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PKC-beta II [pT641] was done on HeLa cells treated with PMA (200nM, 20 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PKC-beta II [pT641] Rabbit Polyclonal Antibody (44964G, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.