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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB43781-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse PKC beta 2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human PKC beta 2 in direct ELISAs. Detects human and mouse PKC beta 2 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P05771- Isotype
- IgG
- Antibody clone number
- 469827
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse PKC beta 2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, CEM human T-lymphoblastoid cell line, and TK-1 mouse T cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PKC beta 2 Monoclonal Antibody (Catalog # MAB43781) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKC beta 2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- PKC beta 2 in Human Spleen. PKC beta 2 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human/Mouse PKC beta 2 Monoclonal Antibody (Catalog # MAB43781) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse/Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC002). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
