PA5-56292

antibody from Invitrogen Antibodies

Targeting: PLEC EBS1, PCN, PLEC1, PLTN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-56292

Provider

Invitrogen Antibodies

Product name

Plectin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: TEIIRQQGLA SYDYVRRRLT AEDLFEARII SLETYNLLRE GTRSLREALE AESAWCYLYG TGSVAGVYLP GSRQTLSIYQ ALKKGLLSAE VARLLLEAQA A

Concentration

0.3 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Plectin was performed by loading 10 µg of WT (lane 1) and PLEC CRISPR KO (lane 2) U2OS cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins were transferred to nitrocellulose membrane and blocked in 5% milk. Ponceau stained transfer of blot is shown. PLEC was detected above 245 kDa using a Plectin polyclonal antibody (Product # PA5-56292) at a dilution of 1:10,000 in 5% BSA in TBST overnight at 4 deg, followed by secondary antibody diluted to 0.2 µg/mL using Goat anti-Rabbit IgG (H+L) HRP antibody (Product # 65-6120). Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Plectin in human cell line U-251 MG shows positivity in cytoplasm, intermediate filaments & focal adhesion sites. Samples were probed using a Plectin Polyclonal Antibody (Product # PA5-56292).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human liver using Plectin Polyclonal Antibody (Product # PA5-56292).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human testis using Plectin Polyclonal Antibody (Product # PA5-56292).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human skeletal muscle using Plectin Polyclonal Antibody (Product # PA5-56292) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human liver, pancreas, skeletal muscle and testis using Plectin Polyclonal Antibody (Product # PA5-56292) (A) shows similar protein distribution across tissues to an independent Plectin Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of Plectin in human pancreas using Plectin Polyclonal Antibody (Product # PA5-56292) shows low expression as expected.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Plectin was performed on U2OS cell lysates. Antibody-bead conjugates were prepared by adding 1 µg of PLEC polyclonal antibody (Product # PA5-56292) with 30 µL of protein A -Sepharose beads and rocked overnight at 4°C. 1 µg of PLEC KO lysate was incubated with antibody-bead conjugate for 2 hrs at 4°C. After multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a PLEC monoclonal antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.